RNAi screening

The high-throughput, genome-scale application of RNA interference to cell-based screens is a powerful method for perturbing gene activities in cultured cells. It is becoming a method of choice for screening drug candidates and target discovery. Appropriately targeted siRNA molecules can be used to provide information about gene function, and the downstream effects on relevant pathways.

Our service includes:

• Guidance with experimental design and choice of appropriate marker set, controls, etc.
• Up-to-date annotation of marker set
• Statistical modelling of data using optimal normalisation methods
• Network and pathway analysis of significant hits
• Biological interpretation in the context of the experiment